Abstract
The agarophyte Gracilaria gracilis is an econom- ically valuable species exploited in Argentina and the car- pospore study is relevant since such spores are used as inoculum for commercial aquaculture. The aims of this work were to describe the carpospore release periodicity and carposporeling development and to evaluate the influ- ence of temperature, irradiance, and day length on carpo- spore release under laboratory conditions. Desiccation, osmotic shock, and spontaneous discharge were compared as sporulation induction methods and the last one was the best method for improved carpospore release (ANOVA: F= 6.4018, P 0.25). Sporulation time course analysis showed a peak during the first week with a total carpospore release at the end of the sixth week of about 2,000 carpospores cystocarp �1 day �1 .
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