Carpesium divaricatum Sieb. & Zucc. Revisited: Newly Identified Constituents from Aerial Parts of the Plant and Their Possible Contribution to the Biological Activity of the Plant.

Natalia Kłeczek,Janusz Malarz,Anna Karolina Kiss,Barbara Michalak,Anna Stojakowska

doi:10.3390/molecules24081614

Abstract

Carpesium divaricatum Sieb. & Zucc. has a long history of use as both a medicinal and a food plant. However, except for terpenoids, its chemical constituents have remained poorly investigated. The composition of hydroalcoholic extract from aerial parts of C. divaricatum was analyzed by HPLC-DAD-MSn, revealing the presence of numerous caffeic acid derivatives that were formerly unknown constituents of the plant. In all, 17 compounds, including commonly found chlorogenic acids and rarely occurring butyryl and methylbutyryl tricaffeoylhexaric acids, were tentatively identified. Fractionation of lipophilic extract from cultivated shoots led to the isolation of 12-oxo-phytodienoic acid (12-OPDA), which is a newly identified constituent of the plant. The compound, at concentrations of 0.5, 1.0, and 2.5 μM, significantly reduced IL-8, IL-1β, TNFα, and CCL2 excretion by lipopolysaccharide (LPS)-stimulated human neutrophils. Reactive oxygen species (ROS) production induced by f-MLP was also significantly diminished in the neutrophils pretreated by 12-OPDA. The newly identified constituents of the plant seem to be partly responsible for its pharmacological activity and elevate the value of C. divaricatum as a potential functional food.

Highlights

The genus Carpesium (Asteraceae, tribe Inuleae), following recent taxonomic studies, comprises species distributed in Asia and southeastern Europe [1]
Some recent reports suggest their implication in prevention of so-called lifestyle diseases, such as diabetes
Some recent reports suggest their implication in prevention of so-called lifestyle diseases, such as diabetes [22,23], cardiovascular and neoplastic diseases [24,25], oxidative-stress-induced cognitive

Summary

Introduction

The genus Carpesium (Asteraceae, tribe Inuleae), following recent taxonomic studies, comprises species distributed in Asia and southeastern Europe [1]. Due to their anti-inflammatory, antipyretic, detoxifying, anti-oedematous haemostatic, and antimalarial properties, have long been used as traditional medicines in China, Japan, and Korea to treat various ailments [2,3]. Whole herbs or aerial parts of the plants are eaten, by local communities, as a seasonal vegetable, functional food, or vitamin source [4,5]. Aerial parts of Carpesium plants are used to prepare brewed beverages or serve as forage [2,5]. The whole plant of Carpesium divaricatum Sieb. Phytochemical studies on specialized metabolites of the plant are almost entirely devoted to terpenoids, especially sesquiterpene lactones, which reportedly possess anti-inflammatory and cytostatic properties [3,6,7]

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