Abstract
The Microbacterium sp. LEMMJ01 isolated from Antarctic soil does not belong to any of the nearest species identified in the RDP database. Under UV radiation (A, B and C wavebands) the survival fractions of Microbacterium sp. cells were much higher compared with wild-type E. coli K12A15. Especially remarkable for an Antarctic bacterium, an expressive resistance against high UV-B doses was observed. The increased survival of DNA repair-proficient E. coli grown overnight added of 0.1 mg/ml or 1 mg/ml of the whole pigment extract produced by Microbacterium sp. revealed that part of the resistance of Microbacterium sp. against UV-B radiation seems to be connected with photoprotection by its pigments. Scanning electron microscopy revealed that UV-A and UV-B ensued membrane alterations only in E. coli. The APCI-MS fingerprints revealed the diagnostic ions for neurosporene (m/z 580, 566, 522, 538, and 524) synergism for the first time in this bacterium by HPLC-MS/MS analysis. Carotenoids also were devoid of phototoxicity and cytotoxicity effects in mouse cells and in human keratinocytes and fibroblasts.
Highlights
Carotenoids are natural pigments that exhibit many biological functions including antioxidant effects
Single colonies of the psychrophilic bacteria were isolated on LB medium after growing at 12 °C for 48 h
Microbacterium sp. isolate LEMMJ01 was selected due to its remarkable vivid colony color and its higher resistance to ultraviolet radiation (UV)-B radiation when compared with the standard strain, E. coli K12A15
Summary
Carotenoids are natural pigments that exhibit many biological functions including antioxidant effects. A pigmented heterotrophic bacterium isolated from Antarctic nitrogen-rich ornithogenic soils was exposed to cycles of simulated ambient solar radiation to select resistant strains bacteria. Isolate LEMMJ01 showed a strong resistance against UV radiation, so that its pigments were extracted, and the carotenoids characterized physico-chemically.
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