Abstract
BackgroundIn this study, renewable tea waste hydrolysate was used as a sole carbon source for carotenoids and lipid production. A novel Rhodosporidium toruloides mutant strain, RM18, was isolated through atmospheric and room-temperature plasma mutagenesis and continuous domestication in tea waste hydrolysate from R. toruloides ACCC20341.ResultsRM18 produced a larger biomass and more carotenoids and α-linolenic acid compared with the control strain cultured in tea waste hydrolysate. The highest yields of torularhodin (481.92 μg/g DCW) and torulene (501 μg/g DCW) from RM18 cultured in tea waste hydrolysate were 12.86- and 1.5-fold higher, respectively, than that of the control strain. In addition, α-linolenic acid production from RM18 in TWH accounted for 5.5% of total lipids, which was 1.58 times more than that of the control strain. Transcriptomic profiling indicated that enhanced central metabolism and terpene biosynthesis led to improved carotenoids production, whereas aromatic amino acid synthesis and DNA damage checkpoint and sensing were probably relevant to tea waste hydrolysate tolerance.ConclusionTea waste is suitable for the hydrolysis of microbial cell culture mediums. The R. toruloides mutant RM18 showed considerable carotenoids and lipid production cultured in tea waste hydrolysate, which makes it viable for industrial applications.
Highlights
In this study, renewable tea waste hydrolysate was used as a sole carbon source for carotenoids and lipid production
Tea waste and tea waste hydrolysate (TWH) The sample of tea waste treated after aqueous phase extraction was examined for quantification of organic content matter, cellulose, hemicellulose, and lignin by the Fujian Academy of Agricultural Sciences, China
There will be a relatively large amount of xylose and arabinose generated in TWH, but a very small amount of byproduct polyaromatic compounds due to the content of hemicellulose and lignin in tea waste, respectively
Summary
Renewable tea waste hydrolysate was used as a sole carbon source for carotenoids and lipid production. A novel Rhodosporidium toruloides mutant strain, RM18, was isolated through atmospheric and room-temperature plasma mutagenesis and continuous domestication in tea waste hydrolysate from R. toruloides ACCC20341. Tea is one of the oldest and most widely consumed aromatic beverages and has immense economic and medicinal value globally [1]. Since the early twentieth century, the annual global consumption of tea has reached millions of tons. The conventional treatment approach for utilizing tea waste was to convert it into biofuels, feedstock for domestic animals or for use as crop fertilizer, both of which have been implemented for many years [2, 3]. The environmental and cost-effective utilization of tea waste has gained significant interest and additional attention in related studies
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