CARNITINE CHLORIDE EFFECT ON FUNCTIONAL STATE OF MITOCHONDRIA IN CONDITIONS OF NITRIC OXIDE SYNTHESIS DEFICIT

Abstract

Aim. To study the effect of carnitine chloride on functional state of mitochondria and endogenous carnitine content in conditions of L-NAME-induced decrease in nitric oxide synthesis (II). Materials and methods. Twenty four Wistar rats were divided into the following groups: the control group, the group with intraperitoneal introduction of L-NAME in the dose of 25 mg/kg for 7 days and the group receiving L-NAME by the analogous scheme against the background of introduction of carnitine chloride in the dose of 300 mg/kg for 21 days. The animals experienced the study of lactate dehydrogenase (LDG), succinate dehydrogenase (SDG), superoxide dismutase (SOD), lactate, nitric oxide (II), carnitine (total, free, bound) and nonetherified fatty acid concentrations in the rat cardiomyocyte mitochondria. Results. L-NAME in the dose of 25 mg/kg led to decrease in NO metabolite (by 21,5 %, p < 0,05) and lactate (by 56 %, p < 0,05) concentrations in myocardiocyte mitochondria; at the same time, it increased nonetherified fatty acid (by 290 %, p < 0,05) and total carnitine (by 162 %, p < 0,05) content as well as activity of all the three measured oxidoreductases in mitochondrial cardiac tissues as compared to the control animal indices (LDG by 160 %, p < 0,05; SDG by 109 %, p < 0,05; SOD by 133 %, p < 0,05). Preliminary introduction of carnitine chloride induced reliable significant growth of SDG (by 163 %, p < 0,05) and SOD (by 376 %, p < 0,05) activity, rise in NO metabolite (by 437 %, p < 0,05) and lactate (by 62 %, p < 0,05) concentrations, as well as to fall in fatty acid (37 %, p < 0,05) and total carnitine (by 35 %, p < 0,05) concentrations and LDG activity (by 50 %, p < 0,05). Conclusions. Preliminary administration of cortinine chloride in the dose of 300 mg/kg before introduction of L-NAME in the dose of 25 mg/kg normalizes carnitine homeostasis, prevents decrease in NO metabolite concentration, declines nonetherified fatty acid concentration and significantly elevates SOD and SDG activity in the rat cardiomyocyte mitochondria.