Abstract
γ-Butyrobetaine hydroxylase (EC 1.14.11.1) is the last enzyme in the biosynthetic pathway ofl-carnitine and catalyzes the formation ofl-carnitine from γ-butyrobetaine, a reaction dependent on α-ketoglutarate, Fe2+, and oxygen. We report the purification of the protein from rat liver to apparent homogeneity, which allowed N-terminal sequencing using Edman degradation. The obtained amino acid sequence was used to screen the expressed sequence tag database and led to the identification of a human cDNA containing an open reading frame of 1161 base pairs encoding a polypeptide of 387 amino acids with a predicted molecular weight of 44.7 kDa. Heterologous expression of the open reading frame in the yeastSaccharomyces cerevisiaeconfirmed that the cDNA encodes the human γ-butyrobetaine hydroxylase. Northern blot analysis showed γ-butyrobetaine hydroxylase expression in kidney (high), liver (moderate), and brain (very low), while no expression could be detected in the other investigated tissues.
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More From: Biochemical and Biophysical Research Communications
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