Abstract
AbstractShort‐chain fatty acids are usually located at positionsn‐3 in natural triglycerides, particulary in dairy fats. As a result, it is extremely difficult to differentiate betweensn‐3 stereospecificity and short‐chain typoselectivity in many lipases and acyltransferases that perform in this way. This ambiguity can be removed through successive use of a chiral triglyceride with a short fatty acid in positionsn‐1 and of its racemic in controlled hydrolysis reactions. After checking that the proposed method effectively confirmed the type of activity of control biocatalysts (Candida cylindracea nonspecific lipase andMucor miehei 1–3 regiospecific lipase), we confirmed thatCarica papaya latex has a strictsn‐3 stereospecificity.
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