Cardiovascular-related peptides influence hypothalamic neurons involved in control of body water homeostasis.

Abstract

The cardiovascular-related peptides, atrial natriuretic peptide (ANP), brain natriuretic peptide (BNP) and endothelin (ET) were originally isolated from the atrium, brain and endothelial cells, respectively. ANP and BNP have hypotensive, natriuretic, diuretic and vasodilator effects. ET has strong vasoconstrictor effects. Centrally applied ANP and BNP attenuate pressure and drinking responses and vasopressin secretion induced by angiotensin II. Similar application of ET increases blood pressure in vivo and vasopressin secretion in vitro. To clarify direct effects of these peptides on neurons in the regions involved in body water homeostasis, extracellular recordings were made from neurons in the supraoptic nucleus (SON) and regions of anteroventral third ventricle (AV3V) of rat hypothalamic slice preparations. ANP and BNP inhibited AV3V neurons, suggesting direct actions of the peptides on drinking. In the SON, these peptides inhibited selectively putative vasopressin neurons but not putative oxytocin neurons, suggesting direct actions of the peptides on vasopressin secretion. We demonstrated that the inhibitory response by ANP and BNP is mediated through a second messenger cGMP system but not cAMP. Contrary to natriuretic peptides, ET excited AV3V neurons but inhibited SON neurons. Roles of ANP, BNP and ET on the central regulatory systems of body water homeostasis, acting as neurotransmitters or neuromodulators, will be discussed.