Abstract
Methods Cardiovascular tissue allografts (CVA) were retrieved from recipients of heart transplants (RHT) and multiorgan donors (MOD) which fulfill donor selection criteria. Processing of CVA was performed in CTB clean room (GMP Class B). Initial processing included surgical dissection, measurements, morphology and functional assessment and collection of quality control samples. CVA were incubated in antibiotic containing medium (vancomycin, lincomycin, polymyxin B sulfate) for 24-48 hours. Then CVA were cryopreserved in solution containing 10% of dimethylsulphoxyde and stored in vapor phase of liquid nitrogen. Cryopreserved tissues remained in the quarantine until all quality control results were collected.
Highlights
The Croatian Cardiovascular Tissue Bank (CTB) was established in June 2011 at University Hospital Centre Zagreb (UHC)
Cardiovascular tissue allografts (CVA) were cryopreserved in solution containing 10% of dimethylsulphoxyde and stored in vapor phase of liquid nitrogen
The cardiovascular tissue bank was successfully established at UHC Zagreb
Summary
The Croatian Cardiovascular Tissue Bank (CTB) was established in June 2011 at University Hospital Centre Zagreb (UHC). Cardiovascular tissues processed in CTB were retrieved and processed according to validated protocols and in compliance with laws and regulations applied in tissue establishments
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