Abstract
Purpose: To study the cardioprotective effects of the total flavonoids from the roots of Polygonum cuspidatum Sieb. et Zucc. (FHZ) on experimental myocardial infarction in mice. Methods: Ultrasonic-assisted extraction of FHZ was optimized by response surface methodology (RSM) to obtain a higher extraction yield. Myocardial infarction (MI) was established by ligation of the left anterior descending (LAD) branch of the coronary artery in mice. Cardiac troponin T (cTnT), creatine phosphokinase (CPK), lactate dehydrogenase (LDH), lipid peroxide (LPO), malondialdehyde (MDA) and superoxide dismutase (SOD) levels in the serum of mice were assessed by enzyme-linked immunosorbent assay. Furthermore, myocardial infarction size (MIS) was examined by Masson’s Trichrome staining on heart tissues. Results: Optimum extraction conditions of FHZ were as follows: an ethanol concentration of 69.03 %, a liquid-solid ratio of 27.14 g/mL and an extraction time of 30.30 min. The obtained extraction conditions were proven to be accurate and reliable. After treatment with FHZ for 3 and 7 days, the serum level of cTnT in MI mice decreased significantly. Also, the serum levels of CPK, LPO, MDA, and LDH were significantly decreased while SOD level increased in MI mice treated with FHZ. Furthermore, after treatment with FHZ for two weeks, the MIS of MI mice decreased ( p < 0.01). Conclusion: RSM is a useful tool to optimize the ultrasonic-assisted extraction conditions for FHZ. Furthermore, FHZ possesses significant cardioprotective effects on experimental myocardial infarction in mice and thus may find application in the clinical management of myocardial infarction. Keywords: Polygonum cuspidatum, Flavonoids, Response surface methodology, Myocardial infarction, Cardioprotective
Highlights
Acute myocardial infarction (AMI) resulting from coronary artery occlusion is a severe disease with a high mortality worldwide [1,2]
Rutin standard was purchased from the National Institute for Food and Drug Control (Beijing, China); Cardiac troponin T, creatine phosphokinase (CPK), lactate dehydrogenase (LDH), lipid peroxide (LPO), malondialdehyde (MDA), and superoxide dismutase (SOD) enzyme-linked immunosorbent assay (ELISA) kits were obtained from Nanjing Jiancheng Bioengineering Institute (Nanjing, China)
Serum was prepared by centrifugation and CPK, LDH, LPO, MDA, and SOD levels were evaluated by ELISA
Summary
Acute myocardial infarction (AMI) resulting from coronary artery occlusion is a severe disease with a high mortality worldwide [1,2]. Using RSM and to investigate the cardioprotective effects of FHZ in mice with experimental myocardial infarction. Optimization of the ultrasound extraction of FHZ was carried out using response surface methodology (RSM) [12] and a three-factor, three-level Box-Behnken design (BBD) consisting of 17 experimental runs was empolyed (Table 2). Male C57BL/6 adult mice (8 weeks old, 20 ± 2 g) were obtained from Shanghai Animal Administration Center (Shanghai, China). The mice were anesthetized intraperitoneally with chloral hydrate and the myocardial infarction (MI) model was established by permanent ligation of the left anterior descending (LAD) branch of the coronary artery [14]. The level of cTnT in the serum of mice was assayed using ELISA kit according to the manufacturer’s instructions. CPK, LDH, LPO, MDA and SOD levels in the serum of mice difference between different groups, and p < 0.05 was considered statistically significant
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