Cardioprotective Effects of an Aminothiazole Compound on Isoproterenol-Induced Myocardial Injury in Mice

Abstract

Dendrodoine analogue (DA), an aminothiazole compound derived from dendrodoine, present in a marine tunicate, has been shown to possess many beneficial properties. This study was aimed to evaluate its cardioprotective effect against isoproterenol (ISO)-induced myocardial damage in mice. Swiss mice were pretreated with DA for 7days and then treated with ISO (5mg/kg BW, for 2 consecutive days). Biochemical assessment of myocardial injury was carried out by measuring marker enzymes, antioxidant enzymes and levels of lipid peroxidation. Histological studies of hearts were also carried out. ISO administration increased the activities of creatine kinase-MB, lactate dehydrogenase and aspartate aminotranferase (AST) in serum. Prior administration of DA restored the levels of these enzymes and the heart coefficient close to normal levels. DA at a concentration of 5mg/kg BW was most effective in reducing AST, and this concentration was used for further studies. DA also gave significant protection against lipid peroxidation in the heart besides restoring histopathological alterations. DA showed significant reactivity towards superoxide radicals. In conclusion our study indicates that DA can protect mouse myocardium against damage and one of the possible reasons behind this protective effect can be attributed to its antioxidant property.