Cardioprotective effect oflow flow perfusion on isolated rat hearts

Abstract

：Objective To compare thecardioprotective effect of low flow perfusion with cold static storage on isolated rathearts. Methods Twenty-four male SD rats were randomly divided into four groups (6 rats ineach group). In two groups the isolated hearts were arrested by infusion of HTK or FWMsolution ( H1 group or F1 group) and then were immersed in 4 ℃ HTK or FWM solution for 8 h, respectively. In the rest two groups, after thehearts were arrested by the two cardioplegias, they were infused continuously by 4 ℃ HTK or FWM solution for 8 h ( H2 group or F2 group) at a pressureof 10 cm H2O. The recovery rate of hemodynamic parameters, the water content, and thelevel of myocardial enzymes of coronary venous sinus drainage were measured. Sequentiallythe myocardial structure was also observed under a light microscope.Results Afterreperfusion, all hearts in F1 group failed to beat. The water content and the leakage ofcreatine kinase MB ( CK-MB), lactate dehydrogenase (LDH) were ( 84. 1 ± 1.2 ) %, ( 16. 2± 7. 4 ) IU/L,(86. 1 ± 10. 6) IU/L in F1 group, higher than those in other three groups(P ＜ 0. 01 ). In H2 group, heartrate, the recovery rate of left ventricular development pressure, rate pressure product,coronary flow and the water content were ( 80. 2 ± 5.3 ) %, (77. 7 ± 3.4 ) %, (63.8 ±3. 1 ) %, ( 54. 6 ± 6. 0 ) %, respectively,which were significantly different from otherthree groups (P ＜ 0. 05). Conclusion The myocardial protective effect of low flow perfusion isbetter than cold static storage. The myocardial protective effect of HTK solution issuperior to FWM solution. Key words: Flow perfusion; Heart preservation; Myocardioprotection