Cardioprotective Effect of the Mixture of Ginsenoside Rg3and CK on Contractile Dysfunction of Ischemic Heart

Abstract

Ginsenosides are one of the most well-known traditional herbal medicines frequently used for the treatment of cardiovascular symptoms in korea. The anti-ischemic effects of the mixture of ginsenoside Rg₃ and CK on ischemiainduced isolated rat heart were investigated through analyses of changes in hemodynamics ; blood pressure, aortic flow, coronary flow, and cardiac output. The subjects in this study were divided into four groups: normal control, the mixture of ginsenoside Rg₃ and CK, an ischemia-induced group without any treatment, and an ischemia-induced group treated with the mixture of ginsenoside Rg₃ and CK. There were no significant differences in perfusion pressure, aortic flow, coronary flow and cardiac output between them before ischemia was induced. The supply of oxygen and buffer was stopped for five minutes to induce ischemia in isolated rat hearts, and the mixture of ginsenoside Rg₃ and CK was administered during ischemia induction. Treatments of the mixture of ginsenoside Rg₃ and CK significantly prevented decreases in perfusion pressure, aortic flow, coronary flow, and cardiac output under ischemic conditions. In addition, hemodynamics (except heart rate) of the group treated with the mixture of ginsenoside Rg₃ and CK significantly recovered 60 minutes after reperfusion compared to the control group (mixture+ischemia vs ischemia - average perfusion pressure: 74.4±2.97% vs. 85.1±3.01%, average aortic flow volume: 49.11±2.72% vs. 59.97±2.93%, average coronary flow volume: 58.50±2.81% vs. 72.72±2.99%, and average cardiac output: 52.47±2.78% vs. 63.11±2.76%, p＜0.01, respectively). These results suggest that treatment of the mixture of ginsenoside Rg₃ and CK has distinct anti-ischemic effects in ex vivo model of ischemia-induced rat heart.