Cardioprotective effect of salvianolic acid B against isoproterenol-induced inflammation and histological changes in a cardiotoxicity rat model

Jun Liu,Huihe Lu,Liang Chen

doi:10.4314/tjpr.v17i11.12

Abstract

Purpose: To investigate the cardioprotective role of salvianolic acid B (SAB-B) against isoproterenol (ISO)-induced cardiotoxicity in a rat model.Methods: A total of 32 male rats were equally divided into four groups as follows: control group given saline only; rats treated with ISO (85 mg/kg; i.p.) for 2 consecutive days (MI model; ISO group); rats pretreated with SAB-B (20 mg/kg, i.p.) prior to exposure to ISO (SAB-B+ISO, treatment group), and rats administrated SAB-B (20 mg/kg, i.p.) for 28 days (SAB-B group).Results: Pre-treatment with SAB-B for 28 days enhanced the levels of various antioxidants (CAT, SOD and GSH) and hemodynamic parameters, viz, systolic blood pressure (SBP), diastolic blood pressure (DBP) and mean arterial pressure (MAP). Likewise, substantial decrease in the levels of lipid peroxidation product (MDA), inflammatory markers (NF-κb, TNF-α, IL-1β and IL-6), heart weight, heartto-body weight ratio, and serum cardiac markers (CPK and LDH) were seen in SAB-B treated rats, relative to ISO-treated group. Rats in SAB-B group had significantly downregulated protein expressions of NF-κb and TNF-α, and significant reduction in histopathological changes (edema and necrosis) after 28 days of treatment.Conclusion: SAB-B possesses potent cardio-protective potential against ISO-induced cardiotoxicity due to its antioxidant, anti-inflammatory and anti-necrotic properties.Keywords: Salvianolic acid B, Isoproterenol, Antioxidants, Inflammatory markers, Cardioprotection

Highlights

Cardiovascular disease (CVD) is one of the major causes of mortality and morbidity globally, and it accounts for 20 % of all deaths
Isoproterenol (ISO) is a non-selective βadrenergic agonist and synthetic catecholamine that imposes considerable oxidative stress on cardiomyocytes due to auto-oxidation of catecholamine; it results in necrosis, edema and apoptosis, and in myocardial injury and myocardial infarction [10,11]
Treatment with SAB-B for 28 days significantly improved the levels of various hemodynamic parameters (p < 0.01)

Summary

INTRODUCTION

Cardiovascular disease (CVD) is one of the major causes of mortality and morbidity globally, and it accounts for 20 % of all deaths. An ISOinduced MI model was used to assess the cardio-protective potential of salvianolic acid B (SAB-B). Previous studies showed that salvianolic acid-A, another phenolic acid from Salvia militiorrhiza exerted cardio-protective activity against isoproterenol-induced myocardial infarction in a rat model [17]. SAB-B exerts vascular protective action against experimentallyinduced acute myocardial infarction in a rat model [18,19]. No studies have carried out to elucidate the cardio-protective effect of salvianolic acid B against isoproterenol-induced myocardial infarction. The present animal study was conducted to investigate the cardioprotective role of SAB-B) against isoproterenol (ISO) induced cardiotoxicity in rat model. Rats administered only saline served as the control group, whereas rats treated with ISO (85 mg/kg, i.p.) for 2 consecutive days (MI model) served as ISO group. All the samples were stored at -80 oC prior to use

Evaluation of hemodynamic parameters

RESULTS

DISCUSSION

CONCLUSION

Conflict of interest