Abstract
Problem statement: The cardio toxicity of Doxorubicin (Dox) has limited its use as an anticancer drug. The aim of present study was to investigate the cardio protective effect of Lagenaria Siceraria (Cucurbitaceous) Fruit Juice (LSFJ) against the cardio toxicity of Dox in rats. Approach: Male wistar rats (250-300 g) were randomly divided into four groups. Group I- was control, Group II- Dox (10 mg kg-1), groups III and IV- Dox + LSFJ (5 and 10 mL kg-1 for 18 days). Dox (10 mg kg-1 iv) was administered in group II, III and IV on day 16. After anesthetizing the animals on the 18th day, Electrocardiogram (ECG) was recorded and blood was investigated for Creatine Kinase-MB is enzyme (CK-MB), Lactate Dehydrogenase (LDH) and Aspartate Aminotransferase (AST) while determination of Superoxide Dismutase (SOD), Reduced Glutathione (GSH), Lipid Per Oxidation (LPO) and histopathology was carried out for heart. Results: Group 3 and 4 of animals showed decreased QT interval (p<0.05) and was non significant in ST interval and heart rate compared to Group 2. Significant decrease in serum CK-MB and AST of group 3 (p<0.05) and group 4(p<0.01) of animals was observed as compared to group II. In group 4 significant increase in the level of GSH (p<0.01) and decrease in MDA content (p<0.001) was observed as compared to group 2, whereas SOD was non-significant. Histopathological study of LSFJ10 mL kg-1 treated group showed protection against myocardial toxicity induced by doxorubicin. Conclusion: It is concluded that Lagenaria siceraria fruit juice possessed cardio protective effect at dose of 10 mL kg-1 against doxorubicin induced cardio toxicity in rats.
Highlights
Lagenaria siceraria (Molina) Standley (Cucurbitaceae) fruit, a commonly used vegetable in India is described as cardiotonic (Deshpande et al, 2008)
Doxorubicin, an anthracycline antibiotic, has a broad antitumor spectrumand has been used against a wide variety of hematopoietic malignancies and solid tumors (Ogura, 2001)
There is paucity of reports on the cardio protective activity of Lagenaria siceraria in Dox-induced cardio toxicity
Summary
Lagenaria siceraria (Molina) Standley (Cucurbitaceae) fruit (bottle gourd), a commonly used vegetable in India is described as cardiotonic (Deshpande et al, 2008). The cardio toxicity of Dox, resulting in a cardiomyopathy with irreversible congestive heart failure and high mortality, is one of the main factors that limit its use. It is hypothesized that antioxidants and iron-chelators may protect the heart against Dox toxicity. Dexrazoxane is an iron chelator and possesses potent antioxidant properties and has been reported to prevent Dox induced cardio toxicity. Due to the high incidence of dexrazoxane-induced myelosuppression, its use has been limited to some advanced stages of malignant disorders (Seifert et al., 1994). Many plants have been reported to possess antioxidant activity. There is paucity of reports on the cardio protective activity of Lagenaria siceraria in Dox-induced cardio toxicity
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