Cardiolipin packing ability studied by grazing incidence X-ray diffraction

Abstract

We report a grazing incidence X-ray diffraction (GIXD) study of pure and mixed Langmuir monolayers of tetramyristoyl cardiolipin (TMCL) and dipalmitoylphosphatidylcholine (DPPC) at 22 °C. The mixing behavior of the two components was investigated at two different surface pressures, 4 and 25 mN m −1. Cardiolipins are found to be in a liquid-condensed (LC) phase at 4 mN m −1 whereas the DPPC molecules appear disordered. At 25 mN m −1, cardiolipins are in a solid phase with their aliphatic chains perpendicular to the interface whereas the DPPC molecules are in the LC phase. At this surface pressure, increasing the amounts of TMCL to DPPC leads to a reduction in tilt angle of the aliphatic chains from nearly 30° for pure DPPC to almost 0° in a 1:1 molar ratio of DPPC and TMCL. At this composition, we also found the closest packing of the aliphatic chains. Further increase of the amount of TMCL does not change the lattice or the tilt and the thermodynamic analysis confirms a partial phase separation. Such a behavior was not observed at 4 mN m −1 where the two phospholipids are miscible at all the compositions studied. Addition of TMCL clearly induces a structuring of the mixed monolayers and increases order by a tight packing in the lipid acyl chains.