CARDIO PROTECTIVE ACTIVITY OF TRINETRA RASA IN EXPERIMENTAL ANIMAL MODEL

Abstract

Cardiovascular diseases are descr ibed under Hridroga in Ayurvedic classics. Trinetra Rasa is a herbo - min e ral formulation explained in the context of Hridroga. The present study wa s aimed to evaluate Cardio protective activity of Trinetra Rasa against Isoprenaline induced cardiac damage in rats and attempt to understand mechanism of its therapeutic effect with resp ect to biochemical markers, ECG and Histopathological changes. Albino rats of Wistar strains of eith er sex between 150 to 250 g , randomly divided into 6 groups , 8 rats in each gro up. Group 1 - Control group given tap water 10 ml/kg, Group 2 - Isoprenaline control group - 100 mg/kg, Group 3 - Standard group given Proprenalol - 10 mg/kg, Group 4 - Honey (Vehicle control/Anupana) - 10 ml/kg (Test 1), Group 5 - Trinetra Rasa in therapeutic dose along with Honey - 750 mg/kg (Test 2) and Group 6 - Trinetra Rasa in 2 times the therapeutic dose along with Honey - 1500 mg/kg ( Test 3 ) were administered according to the body weight of animals by oral route to respective groups at morning hours and continued for 20 days. On 19 th day, ECG was recorded to assess Myocardi al infarction and 1 st dose of Isoprenaline injection (s/c) was given , on 20 th day after 24 hours of 1 st dose, 2 nd dose of Isoprenaline injection was given (s/c ) and ECG was record ed. Biochemical and histopathological assessment was done. In biochemical parameters better effect was observed with Test 2 group but histopathological profile showed good effect at Test 3 group higher dose level. The study provided evidence for presence cardio protectiv e activity of Trinetra Rasa against Isoprenaline induced cardiac damage in rats .