Cardio(lipin) workout beefs up mitochondrial imports

Abstract

Mitochondria depend on imported proteins. Kutik et al. show how a protein keeps this molecular traffic flowing by helping manufacture a key membrane lipid. Figure 1 Without cardiolipin—made with the help of Tam41—transport of proteins into mitochondria like these falters. Almost all of the 1,000 or so different proteins mitochondria need to operate are made elsewhere in the cell. After crossing a mitochondrion's outer membrane, proteins follow one of two routes through the organelle's inner membrane. In the carrier pathway, the protein complex TIM22 shepherds molecules into the inner membrane. The presequence pathway, by contrast, relies on the TIM23 complex. Researchers recently discovered that a protein called Tam41 was required for TIM23 to efficiently import presequence molecules. Whether Tam41 also helped out in the carrier pathway was controversial. Kutik et al. used yeast devoid of Tam41 to answer the question. The researchers measured whether carrier-transported proteins combined into complexes, a sign that they'd arrived at their destination. The team found that in the mutant cells, the proteins often reached the inner membrane but didn't assemble into mature complexes. And the electrical potential that usually exists across the inner membrane dwindled. Those results suggest that not only is the presequence pathway faulty when Tam41 is missing, so is the carrier pathway. The mitochondrial defects resembled those caused by a scarcity of cardiolipin, an inner membrane lipid. Cardiolipin performs many jobs in mitochondria, including stabilizing the protein complexes involved in electron transport. Cardiolipin was almost absent from the mutant cells. Tam41 does not synthesize cardiolipin directly, but helps to fashion one of its precursors, the team found. Next task, the researchers say, is to identify that step. Kutik, S., et al. 2008. J. Cell Biol. .10.1083/jcb.200806048 [PMC free article] [PubMed] [Cross Ref]