Abstract
Objective To assess carcinogen related CYP1A1 and GSTM1 genetic polymorphisms in oral cancer (OC) and oral potentially malignant disorders (OPMD). Study Design One hundred smokers were studied: OC = 30, OPMD = 45 (leukoplakia and oral lichen planus) and 25 controls (Con) by means of biopsy and exfoliative cytology (EC) of the lesion and clinically normal mucosa of a contralateral site. After extracting DNA from EC, polymorphisms study of CYP1A1 using polymerase chain reaction (PCR)-retriction fragment length polymorphism (RFLP) and GSTM1 using conventional PCR were done. Results The CYP1A1 polymorphisms found were (m1/m1), (m1/m2) and (m2/m2). All control showed m1/m1 (wild-type), whereas 80% of OC had (m1/m2) and (m2/m2) genotype. 68% of the OPMD displayed m1/m1. Regarding GSTM1, (no null) wild-type and (null) genotypes were studied; 75% of control had no null, 73% of OC showed null and in OPMD there were no significant differences in the null and no null appearance. Conclusions The modified genotypes of CYP1A1 and GSTM1 could indicate a predisposition for oral cancer development. To assess carcinogen related CYP1A1 and GSTM1 genetic polymorphisms in oral cancer (OC) and oral potentially malignant disorders (OPMD). One hundred smokers were studied: OC = 30, OPMD = 45 (leukoplakia and oral lichen planus) and 25 controls (Con) by means of biopsy and exfoliative cytology (EC) of the lesion and clinically normal mucosa of a contralateral site. After extracting DNA from EC, polymorphisms study of CYP1A1 using polymerase chain reaction (PCR)-retriction fragment length polymorphism (RFLP) and GSTM1 using conventional PCR were done. The CYP1A1 polymorphisms found were (m1/m1), (m1/m2) and (m2/m2). All control showed m1/m1 (wild-type), whereas 80% of OC had (m1/m2) and (m2/m2) genotype. 68% of the OPMD displayed m1/m1. Regarding GSTM1, (no null) wild-type and (null) genotypes were studied; 75% of control had no null, 73% of OC showed null and in OPMD there were no significant differences in the null and no null appearance. The modified genotypes of CYP1A1 and GSTM1 could indicate a predisposition for oral cancer development.
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