Carcinogenic potency of perfluorooctane sulfonate (PFOS) on Syrian hamster embryo (SHE) cells

Abstract

Perfluorooctane sulfonate (PFOS) is the degradation product of many fluoroderivatives and a widespread environmental contaminant. Its persistence, its long half-life in humans and its toxicity explain high concerns on human health side effects in future. PFOS is suspected to be a non-genotoxic carcinogen. In the present work, we assessed carcinogenic potential of PFOS by studying morphological transformation in Syrian hamster embryo (SHE) cells; cell transformation of SHE cells is an in vitro assay recommended by the Organization for Economic Cooperation and Development to detect carcinogens, genotoxic or not. Genotoxicity of PFOS and expression of PPARs genes in SHE cells were also measured. PFOS was shown to induce cell transformation (P<0.05) at non-cytotoxic concentrations (0.2 and 2μg/mL) (P≤0.01). No genotoxic effect was recorded in the range of PFOS concentrations tested (2×10(-4) to 50μg/mL) using the single-cell gel electrophoresis (comet) assay after 5 and 24h of exposure. The expression of PPARs genes was measured by qPCR within the first 24h and after 7days of PFOS treatment. Results indicated an increased expression of ppar-β/δ isoform as early as 24h. After 7days, the increase of ppar-β/δ mRNA was significant at the concentrations inducing cell transformation (0.2 and 2μg/mL), while overexpression of ppar-γ and ppar-α did not closely relate to effective concentrations. The results indicate that PFOS behave as a non-genotoxic carcinogen and impacted PPARs genes. Its cell transforming potential paralleled an increased expression of ppar-β/δ.