Carcass and fatness traits of Central Javanese local ducks based on Lipoprotein Lipase (LPL) and Perilipin (PLIN) genes

Abstract

This research is an exploratory study that determines the genotypes of carcass and fatness traits in Central Javanese local ducks based on the lipoprotein lipase (LPL) and perilipin (PLIN) genes. A 35 ducks were sampled from seven local ducks in Central Java. The duck’s DNA was isolated from its feathers. PLP and PLIN genes were amplified by PCR method using specific primers. In the RFLP analysis, PCR products were digested with the restriction enzymes MvaI (BstNI) (5’CC/WGG) and Bsp1286. (SduI) (GDGCH/C), for LPL and PLIN gene, respectively. The results of PCR-RFLP analysis on local ducks in this study showed a monomorphic in exon 2 of the PLIN gene and polymorphic in exon 5 of the LPL gene. Based on the LPL gene, as many as 29 ducks (82.85%) had GG genotypes, 3 ducks (8.57%) had AG types and 3 ducks (8.47%) had AA types. Both PLIN and LPL genotypes were clustered the Central Javanese local ducks into 3 haplotypes (A-C). The duck samples were dominated by haplotype A (82.85%) followed by haplotype B (8.57%) and haplotype C (8.57%). Results showed that 82.85% of Central Javanese local ducks genetically had a good of fatness traits but a low of carcass traits.