Carboxyl-terminal residues of fibrinogen and fibrin

Abstract

The carboxyl-terminal residues of bovine fibrinogen, fibrin and the chain peptides, α(A), β(B) and γ, isolated from it were determined by hydrazinolysis, digestion with carboxypeptidase and selective tritium labelling. The C-terminal ends of both fibrinogen and fibrin were identified as proline and valine, in the ratio of approximately 1:2. Proline was identified as the C-terminus of the α (A)-chain isolated, and C-terminal valine was found on both the β(B)- and γ-chain. On hydrazinolysis after selective tritium labelling of fibrinogen radioactive C-terminal valine was obtained.