Abstract
Abstract: The present study explores conditions to activate the carboxyl group before the coupling of C-terrninal amino acid with Trimethylsilyl -isothiocyanate (TMS-ITC). Protected amino acids and different peptides were used as model and 1,3'-Dicyclohexylcarbodiimide (DCC) plus 1-Hydroxybenzotriazole (HOBt) and 2-(lH-Benzotriazol-l-y)- 1,1,3,3-tetramethyluronium tetrafluoroborate (TBTU) plus HOBt were used as carboxyl activators. The coupling reaction of activated AA with TMS-ITC was temperature dependent and the best yield (100 % of coupling) was obtained at 70°C, when DCC or TBTU both with HOBt were used as activating reagents.
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