Abstract
1. Carbonic anhydrase levels were determined for the blood, gill and intestine of the flounder Platichthys flesus acclimated to seawater and to freshwater. 2. Mean levels of enzyme activity in blood were not significantly different from zero although significant levels were found in gills and intestines. 3. There was no significant difference between seawater and freshwater adapted fish in the enzyme levels of the gills or, after allowing for fish weight, of the intestines. 4. Intestinal carbonic anhydrase activity was significantly lower than gill activity in freshwater adapted, but not in seawater adapted, fish. 5. The possible involvement of carbonic anhydrase in chloride transport is discussed in the light of these observations.
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