Carbonic anhydrase activity in the peripheral nervous system of rat: the enzyme as a marker for muscle afferents

Abstract

The distribution of carbonic anhydrase (CA) activity was determined histochemically using Hansson's cobalt phosphate method in cross-sections of peripheral nerves from rats. As the studied nerves contain either efferent, proprioceptive or exteroceptive myelinated fibres, our survey particularly focused on the question whether CA-reactive nerve fibres are functionally related. Intense CA activity was detected in all large diameter (8–12 μm) muscle afferents. The amount of similarly reactive cutaneous afferents was negligibly low (3.6%). Efferent fibres displayed only weak CA activity, which was confined to the small myelinated fibres (3–6 μm). Moderate staining could be assessed in medium-sized (4–11 μm) proprioceptive fibres. The same reactivity occurred in a sizeable percentage (11.4%) of exteroceptive afferents. Their diameters ranged from 4 to 11 μm. These results indicated, that high enzyme activity is found predominantly in large-calibre proprioceptive afferents, which according to Hunt's classification were identified as group IA and IB fibres. Further confirmation for our observations was obtained by demonstrating high levels of enzyme activity in primary nerve endings of muscle spindles (IA fibres) and in axon terminals of Golgi tendon organs (IB fibres) constantly. Finally possible functions for neuronal CA are discussed with respect to its high activity in a functionally related subpopulation of nerve fibres.