Carbonate precipitation and nitrogen fixation in AMG (Artificial Martian Ground) by cyanobacteria

Abstract

This article describes experiments performed to study the survival, growth, specific adaptations and bioremediation potential of certain extreme cyanobacteria strains within a simulation of the atmospheric composition, temperature and pressure expected in a future Martian greenhouse. Initial species have been obtained from Mars-analogue sites in Georgia. The results clearly demonstrate that specific biochemical adaptations allow these autotrophs to metabolize within AMG (Artificial Martian Ground) and accumulate biogenic carbon and nitrogen. These findings may thus contribute to the development of future Martian agriculture, as well as other aspects of the life-support systems at habitable Mars stations. The study shows that carbonate precipitation and nitrogen fixation, performed by cyanobacterial communities thriving within the simulated Martian greenhouse conditions, are cross-linked biological processes. At the same time, the presence of the perchlorates (at low concentrations) in the Martian ground may serve as the initial source of oxygen and, indirectly, hydrogen via photo-Fenton reactions.Various carbonates, ammonium and nitrate salts were obtained as the result of these experiments. These affect the pH, salinity and solubility of the AMG and its components, and so the AMG's scanty biogenic properties improved, which is essential for the sustainable growth of the agricultural crops. Therefore, the use of microorganisms for the biological remediation and continuous in situ fertilization of Artificial Martian Ground is possible.