Abstract
Carbon sources representative of various entry pathways of carbon metabolism viz. fructose, gluconate, glucose and TCA cycle intermediates were used as energy sources to study the invivo nitrate reductase activity in whole cells of A brasilense ATCC 29145. Enzyme activity was linear when using cells grown and immediately assayed using nutrient broth. Organic acids such as malate, succinate, pyruvate, and gluconate were all effective as sources of energy for the reduction of nitrate to nitrite under aerobic conditions. Among the carbohydrates, glucose and sucrose could not support nitrate reduction, while fructose was able to supply energy after a lag of 10–20 minutes. This lag was also observed when using organic acids.
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