Abstract
Sixty-eight Actinobacillus pleuropneumoniae strains were isolated from porcine acute pleuropneumonia cases from different parts of Hungary between 2000 and 2014. A total of 41 isolates were identified as A. pleuropneumoniae bio-type I and 27 strains as biotype II based on cultural, morphological and biochemical characteristics. The aim of this study was to evaluate metabolic fingerprinting in the species-level identification of A. pleuropneumoniae isolates. Utilisation of carbon sources by these field isolates and six reference strains was characterised by the Biolog system (GN2 Microplate, MicroLog3 Version 4.20.05 software). Twenty-nine field strains were correctly identified by the Biolog system as A. pleuropneumoniae, 36 strains as A. lignieresii, two strains as H. paraphrohaemolyticus and one strain as A. equuli after 24 h of incubation. Among the six A. pleuropneumoniae reference strains the Biolog system identified one strain as A. pleuropneumoniae, four as A. lignieresii and one as H. paraphrohaemolyticus. There was no correlation between biotypes and serotypes of A. pleuropneumoniae and the carbon source utilisation pattern and species identification by the Biolog system. our data indicate that the efficacy of the Biolog system used here could be improved by including phenotypes of more A. pleuropneumoniae strains representing a wider geographical occurrence into the database.
Highlights
Sixty-eight Actinobacillus pleuropneumoniae strains were isolated from porcine acute pleuropneumonia cases from different parts of Hungary between 2000 and 2014
Twenty-nine out of the 68 field isolates were identified by the Biolog system as A. pleuropneumoniae, 36 strains as A. lignieresii, two strains as H. paraphrohaemolyticus, and one strain as A. equuli
Our results show that identification of the primary pig pathogen A. pleuropneumoniae based on carbon source utilisation using the Biolog system has only limited value due to the high similarity of A. pleuropneumoniae and A. lignieresii
Summary
Sixty-eight Actinobacillus pleuropneumoniae strains were isolated from porcine acute pleuropneumonia cases from different parts of Hungary between 2000 and 2014. Actinobacillus pleuropneumoniae is one of the most important bacterium species causing respiratory disease in swine all over the world. Acute haemorrhagic-necrotic pneumonia with fibrinous pleuritis can usually be seen among 12- to 16-week-old pigs, but the acute or peracute form can be recognised in all ages, while the chronic form generally develops after the disappearance of acute signs Both forms can cause huge economic losses (Marsteller and Fenwick, 1999; Christensen and Bisgaard, 2004; Gottschalk, 2012). There are six members of the Actinobacillus genus which are nowadays recognised as significant causes of diseases in animals: A. pleuropneumoniae, A. suis, A. lignieresii, A. equuli, A. seminis, and A. capsulatus (Rycroft and Garside, 2000). Actinobacillus muris, A. hominis and A. ureae are species of little veterinary impact (Christensen and Bisgaard, 2004)
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