Carbon Nanotubes for the Development of Glucose Biosensors Based on Gold Electrodeposition

Abstract

Superscript text Subscript textOur study is to develop a general design of biosensors based on vertically aligned Carbon Nanotube (CNT) arrays. Glucose biosensor is selected as the model system to verify the design of biosensors. In the preliminary design, glucose oxidase (GOx) is attached to the walls of the porous alumina membrane by adsorption. Porous highly ordered anodized aluminum oxide (AAO) are used as templates. Deposited gold on both sides of template surfaces serve as a contact and prevent non-specific adhesion of GOx on the surface. In order to find out optimized thickness of gold coating, the redox reaction in([Fe(CN)6]3-/[Fe(CN)6]4-system is monitored by CV. Subsequently, enzymatic redox reaction in glucose solutions is also attempted by CV. We expect protein layers with GOx from a conductive network. To take advantage of the attractive properties of CNTs, the design of enzyme electrodes is modified by attaching CNT onto the sidewalls of AAO template nanopores and then immobilizing GOx to the sidewalls and tips of CNTs. Cobalt is used as a catalyst to fabricate CNTs. As a result, MWCNTs are fabricated inside the AAO templates by CCVD.