CARBON ISOTOPE DISCRIMINATION IN MANGO LEAVES UNDER SALT STRESS

Juliana Cristina Veccelli,Dalmo Lopes De Siqueira

doi:10.1590/0100-29452017826

Juliana Cristina Veccelli, Dalmo Lopes De Siqueira

Open Access

https://doi.org/10.1590/0100-29452017826

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Abstract

ABSTRACT The aim of this study was to evaluate the response of mango plants ( L) ‘Mangifera indicaTommy Atkins’ cultivar to different NaCl concentrations. In this experiment, plants were grown in pots with washed sand and nutrient solution with 0, 10, 20 and 30 mM NaCl for 120 days. After this period, young leaves from the last growth spurt were harvested in a randomized block design, dried at 65 °C for 72 h and ground in a ball mill. Samples were dried again in an oven at 60 °C for 24 h and then the relative abundance of 13C and 12C isotopes was determined using mass spectrometer Sercon 20-20 isotope ratio model. Data were used to determine the carbon isotopic discrimination (?). It was concluded that ‘Tommy Atkins’ mango trees submitted to 30 mmol L-1 NaCl discriminated less 13C, which may be an indication of salt stress in these plants.

Highlights

RESUMO – Com o objetivo de estudar a resposta de plantas de mangueira (Mangifera indica L.) ‘Tommy Atkins’ a diferentes concentrações de NaCl, um experimento foi conduzido, sendo as plantas cultivadas em vasos com areia lavada e solução nutritiva, com 0; 10; 20 e 30 mM de NaCl por 120 dias
Salt stress inhibits plant growth by osmotic effect, restricting water availability due to the toxic effect of specific ions such as Na+ and Cl(FLOWERS; FLOWERS, 2005) and nutritional disorder may occur (AL-YASSIN, 2004), which can lead to morphological changes that are more marked than the osmotic effect (EPSTEIN; BLOOM, 2006)
There are two carbon isotopes (C) that are stable and are found in nature in defined proportions, which remain relatively stable in any organic residue: 12C is the lightest and presents the largest proportion in relation to total C in nature (98.89%), and 13C accounts for only 1.11% of total C (ALVES et al, 2005)

Summary

Introduction

RESUMO – Com o objetivo de estudar a resposta de plantas de mangueira (Mangifera indica L.) ‘Tommy Atkins’ a diferentes concentrações de NaCl, um experimento foi conduzido, sendo as plantas cultivadas em vasos com areia lavada e solução nutritiva, com 0; 10; 20 e 30 mM de NaCl por 120 dias. Isotopic composition analyses of C are made by measuring the 13C / 12C ratio of samples against an international standard, and the result is expressed in terms of δ13C difference from the standard.

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