Carbon investment into mobilization of mineral and organic phosphorus by arbuscular mycorrhiza

Alberto Andrino,Leopold Sauheitl,Stefan Burkart,Georg Guggenberger,Jens Boy

doi:10.1007/s00374-020-01505-5

Alberto Andrino, Leopold Sauheitl + Show 3 more

Open Access

https://doi.org/10.1007/s00374-020-01505-5

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Abstract

To overcome phosphorus (P) deficiency, about 80% of plant species establish symbiosis with arbuscular mycorrhizal fungi (AMF), which in return constitute a major sink of photosynthates. Information on whether plant carbon (C) allocation towards AMF increases with declining availability of the P source is limited. We offered orthophosphate (OP), apatite (AP), or phytic acid (PA) as the only P source available to arbuscular mycorrhiza (AM) (Solanum lycopersicum x Rhizophagus irregularis) in a mesocosm experiment, where the fungi had exclusive access to each P source. After exposure, we determined P contents in the plant, related these to the overall C budget of the system, including the organic C (OC) contents, the respired CO2, the phospholipid fatty acid (PLFA) 16:1ω5c (extraradical mycelium), and the neutral fatty acid (NLFA) 16:1ω5c (energy storage) at the fungal compartment. Arbuscular mycorrhizal (AM) plants incorporated P derived from the three P sources through the mycorrhizal pathway, but did this with differing C-P trading costs. The mobilization of PA and AP by the AM plant entailed larger mycelium infrastructure and significantly larger respiratory losses of CO2, in comparison with the utilization of the readily soluble OP. Our study thus suggests that AM plants invest larger C amounts into their fungal partners at lower P availability. This larger C flux to the AM fungi might also lead to larger soil organic C contents, in the course of forming larger AM biomass under P-limiting conditions.

Highlights

Phosphorus is the most limiting nutrient for plant growth after nitrogen (N), especially in soils from tropical ecosystems, where secondary minerals immobilize P (Johnston et al 2019)
We showed that arbuscular mycorrhiza (AM) plants took up P derived from orthophosphate (OP), apatite (AP), and phytic acid (PA) exclusively via the mycorrhizal pathway with different C-P trading costs
We identified different P acquisition strategies to mobilize the less available P sources PA and AP

Summary

Introduction

Phosphorus is the most limiting nutrient for plant growth after nitrogen (N), especially in soils from tropical ecosystems, where secondary minerals immobilize P (Johnston et al 2019). Almost 80% of plant species establish symbiotic associations with arbuscular mycorrhizal fungi (AMF) (Smith and Read 2008). 2009), explores a large volume of soil and does this at a lower cost as compared with roots Such low-cost modes of P acquisition are expected to be favored by natural selection and during the assembly of plant communities by competition (Raven et al 2018). The AMF are able to mobilize the otherwise poorly accessible phosphate ions beyond the P depletion zone (Osorio et al 2017), and are responsible for nearly 80% of the plant P uptake (Douds and Johnson 2007)

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