Carbon flow into the end-products of photosynthesis in short and long incubations of a natural phytoplankton population

Abstract

During a cruise to the eastern Canadian Arctic (Northern Baffin Bay) in the summer of 1980, we took advantage of the 24-h photoperiod to conduct a 32-h time course experiment of 14C accumulation under natural solar radiation. The degree of non-linearity in the time course was judged against a time-dependent curve of radioactivity constructed by cumulatively adding the amount of 14C taken up in sequential short (2 h) incubations of plankton held in a replicate bottle but left unlabelled until removed for assay. Departure from linearity was due first to decreasing rates of 14C incorporation into polysaccharides and then into lipids. There was a close correspondence between 14C incorporation into proteins in the 32-h incubation and in the sequence of short incubations. These observations are consistent with patterns in utilization of photosynthetic end-products established from laboratory studies of unicellular algal cultures. Based on parallel or independent control experiments, it was judged that complicating factors such as diel light changes, nitrogenous nutrient exhaustion, bottle size effects or inhibitory conterminants in NaH14CO3 stock solutions would not seriously affect our interpretation that non-linearity resulted from catabolic loss of radiocarbon.