Carbon flow conversion induces alkali resistance and lipid accumulation under alkaline conditions based on transcriptome analysis in Chlorella sp. BLD

Abstract

Alkaline environments are abundant globally and cause damage to most organisms, while some microalgae can grow well and accumulate lipids under those conditions. Here the mechanisms of alkali resistance and lipid accumulation in the alkaliphilic microalgae Chlorella sp. BLD were explored using physiological-biochemical and transcriptome analysis. When cultivated at alkaline pH, Chlorella sp. BLD exhibited good alkali-resistance ability and increased biomass (0.97 g L−1). The biochemical composition of Chlorella sp. BLD changed significantly (lipid content increased 39% and protein content decreased 19.5%) compared with pH 7.5. Through transcriptome analysis, we found that pathways related to carbon metabolism such as photosynthesis, glycolysis, and the TCA cycle were significantly regulated under alkaline conditions. Genes that encoding the key enzyme in carbon-related metabolism such as Rubisco, AMY, PK, ME, CS, ACAT, KAS, and DGAT were identified. Transcriptional regulation of these genes results in carbon flow switching from starch and protein to cell wall metabolism, organic acid synthetic and lipid accumulation in response to alkaline conditions. These results reveal the alkali resistance mechanism of Chlorella sp. BLD and provide a theoretical basis for microalgae oil production under alkaline conditions.