Carbon Dots Improve Nitrogen Bioavailability to Promote the Growth and Nutritional Quality of Soybeans under Drought Stress.

Abstract

The inefficient utilization of nitrogen (N) in soil and drought stress seriously threatens agricultural and food production. Herein, soil application of carbon dots (CDs, 5 mg kg-1) promoted the growth and nutritional quality of soybeans by improving N bioavailability, which was beneficial to alleviate the economic losses caused by drought stress. Soil application of CDs enhanced the N-fixing ability of nodules, regulated rhizosphere processes, and ultimately enhanced N and water uptake in soybeans under drought stress. Compared to control (drought stress), the application of CDs under drought stress enhanced soybean nitrogenase activity by 8.6% and increased N content in soybean shoots and roots by 18.5% and 14.8%, respectively. CDs in soil promoted the secretion of root exudates (e.g., organic acids, fatty acids, and polyketides) and regulated beneficial microbial communities (e.g., Proteobacteria, Acidobacteria, Gemmatimonadetes, and Actinobacteria), thus enhancing the N release from soil. Besides, compared to control, the expression of GmNRT, GmAMT, GmLB, and GmAQP genes in roots were upregulated by 1.2-, 1.8-, 2.7-, and 2.3-fold respectively, implying enhanced N transport and water uptake. Furthermore, the proteins, fatty acids, and amino acids in soybean grains were improved by 3.4%, 6.9%, and 17.3%, respectively, as a result of improved N bioavailability. Therefore, CD-enabled agriculture is promising for improving the drought tolerance and quality of soybeans, which is of significance for food security in facing the crisis of global climate change.