Abstract
Summary A growth promoting effect of carbon dioxide was observed when Catharanthus roseus suspensions were cultured in a 2 % C0 2 -enriched environment. No difference in the developmental pattern of PEP carboxylase capacity ( in vitro measurements) was noticed for the tested culture conditions. In vivo measurements showed that the rate of CO 2 fixation was dependent on the CO 2 partial pressure in the culture medium. Enhanced supply of carbon dioxide resulted in an increase of CO 2 fixation by cells during the entire growth cycle. Analysis of label distribution among the products of ( 14 C)O 2 fixation demonstrated that this non-autotrophic carbon assimilation was consistent with an anaplerotic pathway. The relationship between the dark CO 2 fixation process and the enhanced growth thus obtained is discussed.
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