Carbon costs of nitrate reduction in broad bean (Vicia fabaL.) and pea (Pisum sativumL.) plants

Abstract

AbstractThe present study aimed at the assessment of carbon (C) costs for nitrate reduction by measuring the additional CO2amounts released from roots of nitrate‐fed plants in comparison with urea‐fed plants. Only roots were suitable for these determinations, because nitrate reduction in illuminated shoots is fed nearly exclusively by reducing equivalents coming directly from photosynthetic processes. Therefore, in a first experiment, the sites of nitrate reduction were determined in nodule‐free broad bean (Vicia fabaL.) and pea (Pisum sativumL.) plants grown in pots filled with quartz sand and supplied with KNO3. The extent of nitrate reduction in the various plant organs was determined by measuringin vitronitrate reductase activity andin situ15NO$ _3^- $reduction. Only between 9% and 16% of nitrate were reduced in roots of German pea cultivars, whilst 52% to 65% were reduced in broad bean roots. Therefore, C costs of the process could be determined only in broad bean, using an additional pot experiment. The C costs amounted to about 4.76 mol C (mol N)–1or 4 mg C (mg N)–1, similar to those measured earlier for N2fixation. The high proportion of nitrate reduction in shoots of pea plants implies that only very little C is required for this nitrate fraction. This can explain the better root growth of nitrate‐nourished pea plants in comparison with N2‐fixing organisms, which need C compounds for N2reduction in roots. Moreover, a different availability of photosynthates in roots of plant genotypes could explain physiologically the occurrence of “shoot and root reducers” in nature.