Abstract
The first aim of this study was to fabricate vertically aligned multiwalled carbon nanotubes (MWCNTs). MWCNTs were successfully prepared by using plasma enhanced chemical vapour deposition. Further, three carbon composite electrodes with different content of carbon particles with various shapes and sizes were prepared and tested on measuring of nucleic acids. The dependences of adenine peak height on the concentration of nucleic acid sample were measured. Carbon composite electrode prepared from a mixture of glassy and spherical carbon powder and MWCNTs had the highest sensitivity to nucleic acids. Other interesting result is the fact that we were able to distinguish signals for all bases using this electrode.
Highlights
In the last two decades, nanomaterials in the form of nanotubes and nanowires have begun to be reported as promising materials for wide field of applications [1,2]
The reproducible nanostructured electrode surface could be fabricated using lithography as a common tool for microelectronics devices implementation [5], anodization process for nanorods or nanotubes creation [6,7]. One of these techniques is the creation of vertically aligned multiwalled carbon nanotubes (MWCNTs) grown directly on the surface using chemical vapour deposition (CVD) [8]
It is known that that cytosine, adenine, thymine and guanine give signals at carbon electrodes [15,16,17]. We found that both the nucleic acid samples gave all four signals corresponding to single bases at the tested electrodes
Summary
In the last two decades, nanomaterials in the form of nanotubes and nanowires have begun to be reported as promising materials for wide field of applications [1,2] Such materials could be used for fabrication of miniaturized electrodes. The reproducible nanostructured electrode surface could be fabricated using lithography as a common tool for microelectronics devices implementation [5], anodization process for nanorods or nanotubes creation [6,7]. One of these techniques is the creation of vertically aligned multiwalled carbon nanotubes (MWCNTs) grown directly on the surface using chemical vapour deposition (CVD) [8]. The aim of this study was to fabricate MWCNTs and further to test the particles as a part of carbon composite electrodes with commercial carbon particles on detection of nucleic acids
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