Abstract
A self-supported nanoporous cobalt phosphide (CoP) nanowire arrays on the carbon cloth (CC) as an active matrix was fabricated and utilized as an interface for the construction of hemoglobin (Hb)-based biosensors. This matrix was wrapped in a thin aluminum sheet by handmade. Moreover, the electrochemistry and electrocatalytic properties of Hb were investigated on such a matrix. The results revealed that the self-supported nanoporous CoP nanowire arrays on CC significantly enhanced the electrochemical responses of Hb. In the meantime, the immobilized Hb remained its good bioactivity and high catalytic activity to hydrogen peroxide (H2O2). This matrix is thus a suitable platform to develop highly sensitive enzyme-based biosensor, which has the linear range of H2O2 concentration from 2 to 2670 μM with a detection limit of 0.7 μM (S/N = 3). Consequently, it is desirable to explore the possibility of developing a kind of simpler and cheaper matrix electrode.
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