Carbon catabolite repression of the maltose transporter revealed by X-ray crystallography

Abstract

SummaryEfficient carbon utilization is critical to the survival of microorganisms in competitive environments. To optimize energy usage, bacteria have developed an integrated control system to preferentially uptake carbohydrates that support rapid growth. The availability of a preferred carbon source such as glucose represses the synthesis and activities of proteins necessary for the transport and metabolism of secondary carbon sources. This regulatory phenomenon is defined as carbon catabolite repression (CCR)1. In enteric bacteria, the key player of CCR is a component of the glucose-specific phosphotransferase system, enzyme IIA (EIIAGlc)1,2. It is known that unphosphorylated EIIAGlc binds and inhibits a variety of transporters when glucose is available1,2. However, understanding the underlying molecular mechanism has been hindered by the complete absence of structures for any EIIAGlc-transporter complexes. Here, we present the 3.9 Å crystal structure of EIIAGlc in complex with the maltose transporter, an ATP-binding cassette (ABC) transporter. The structure shows that two EIIAGlc molecules bind to the cytoplasmic ATPase subunits, stabilizing the transporter in an inward-facing conformation and preventing the structural rearrangements necessary for ATP hydrolysis. We also show that the half maximal inhibitory concentrations of the full-length EIIAGlc and an N-terminal truncation mutant differ by 60 fold, consistent with the hypothesis that the N-terminal region, disordered in the crystal structure, functions as a membrane-anchor to increase the effective EIIAGlc concentration at the membrane3,4. Together these data suggest a model of how the central regulatory protein EIIAGlc allosterically inhibits maltose uptake in E. coli.