Abstract
The utilization of 28 nitrogen compounds and 24 carbohydrates by 23 strains ofMicromonospora was examined after growth on a chemically defined medium. Inorganic ammonium salts served as better nitrogen sources than nitrate salts, and most of the organisms metabolized acidic or basic amino acids more extensively than neutral ones, except for l-serine. Utilization of carbohydrates in the defined medium did not always correspond to that observed when Luedemann’s medium was used as a basal medium. Experiments using p-nitrophenylglycosides indicated that α-glucosidase, β-glucosidase, β-galactosidase and β-N-acetylglucosaminidase were present in all the strains. The diagnostic properties found in this study are discussed in terms of the classification of Micromonospora species.
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