Abstract
Mycelial growth of an isolate of T. bakamatsutake was tested in media with C/N ratio ranging from 0 to 50 and with 32 carbon and 12 nitrogen sources. The isolate grew best at the C/N ratio of 30. It utilized the monosaccharides d-glucose, d-mannose, and d-fructose, the disaccharide trehalose, and polysaccharide pectin among the carbon sources; and yeast extract, l-glutamic acid, and ammonium compounds among the nitrogen sources. The growth of ten isolates and secretion of gluconic and oxalic acids were compared in d-glucose, trehalose, and pectin media. The utilization of d-glucose, trehalose, and pectin differed among the ten isolates, but all the isolates secreted gluconic acid in the d-glucose media and oxalic acid in the pectin media.
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