Abstract
Sucrose, glucose and fructose are degraded in the Gram-negative bacterium Zymomonas mobilis via an anaerobic version of the Entner-Doudoroff pathway, to an equimolar mixture of ethanol and carbon dioxide. Sucrose is split extracellularly into glucose and fructose (or levan). The two sugars are transported into the cell via facilitated diffusion (uniport). A periplasmic enzyme, glucose-fructose oxidoreductase, provides the novel compatible solute, sorbitol, to counteract detrimental osmotic stress. Carbon flux and its regulation, and branches into anabolic pathways are discussed together with recent approaches to broaden the substrate range of the bacterium.
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