Abstract
The paper describes the detection of carbohydrate- lectin interaction on graphene-on-metal surface plasmon res- onance (SPR) interfaces. Graphene-coated gold-based SPR interfaces were formed through the transfer of large-area graphene grown by chemical vapor deposition (CVD) on polycrystallineCufoils.Themethodallowed successfultrans- fer of single- and double-layered graphene sheets onto the SPR interfaces in a reproducible manner. Functionalization of the graphene interface with mannose was achieved by simple immersion into a mannose aqueous solution (100 mM), resulting in noncovalent interactions between the aromatic ring structure of graphene and mannose. The utility of the carbohydrate-modified graphene-on-gold interface for the se- lective and sensitive detection of carbohydrate-lectin interac- tions was demonstrated using model lectins from Lens culinaris (LC )a ndTriticum vulgaris (TV). While LC lectin binds specifically to mannopyranoside units, TV lectin has an affinity for N-acetyl glucosamine and sialic acid residues.
Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
