Abstract
In this study, a general method for fabricating glycosylated conductive polymer (CP) biointerface was demonstrated for a label-free electrochemical biosensor. A 3-(3-azidopropoxy) thiophene monomer was designed and synthesized. By electrochemically polymerizing this monomer in ionic liquid [Bmim][BF4], a unique conductive polymer was formed, allowing grafting of lactose or ferrocene via post-polymerization Cu(I)-catalyzed click reaction. X-ray photoelectron spectra validated the covalent 1, 2, 3-triazole ring formation during this click functionalization. SEM study showed an increasing of the crystallinity of the CPs after click functionalization with lactose or ferrocene. The binding of the fabricated lactosylated polythiophene biointerface with Erythrina Cristagalli lectin (ECL) was characterized with differential pulse voltammetry, showing excellent sensitivity and selectivity for ECL analysis. This study illustrated a new glycosylated CP biointerface design and fabrication method that can be applied for developing real-time and label-free electrochemical biosensor to study protein-carbohydrate interactions for protein analysis.
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