Abstract
Neutral and acidic glycolipids were extracted from chicken hearts. The neutral and acidic compounds were separated by preparative thin-layer chromatography into eight and two fractions, respectively. Total hydrolysis by mineral acid, permethylation analysis, and sequential cleavage with exoglycosidases showed the presence of glycolipids that belong to the globo- and gala-oligosaccharide series, i.e., the monohexosylceramides Glc-Cer and Gal-Cer, the dihexosylceramides Galβ1–4Glc-Cer and Galα1–4Gal-Cer, the tetrahexosylceramides GalNAcβ1–3Galα1–4Galβ1–4Glc-Cer and GalNAcα1–3GalNAcβ1–3Galα1–4Gal-Cer (III 3GalNAcα-Ga 3Cer) and four subfractions of the Forssman glycolipid GalNAcα1–3GalNAcβ1–3Galα1–4Galβ1–4Glc-Cer. With the notable exception of III 3GalNAcα1-Ga 3Cer, all glycolipids with terminal GalNAcα1–3GalNAc1 reacted on thin-layer chromatograms with a monoclonal anti-Forssman antibody. The major components of the acidic fraction glycolipids were characterized as the lactosebased gangliosides G lac1 (GM3) and G lac2 (GD3).
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