Abstract
A simple and sensitive LC method for the determination of amino compounds, using carbazole-N-acetyl-N-hydroxy-succinimide (CAHS) as condensation agent, has been developed.A mixture of amines is treated with CAHS, in the presence of triethylamine catalyst in non-aqueous acetonitrile or in the presence of 0.2 M borate buffer at pH 8.0–9.0 in 40% (v/v) of acetonitrile solution, to give quantitative yields of amides. Emission maximum for the derivatized amines is 365 nm (λex 335 nm). Studies on derivatization conditions indicate that amines react very fast with CAHS under proposed conditions. The method, in conjunction with a multi-step gradient, offers baseline resolution of common amine derivatives on a reversed-phase C18 column. Derivatization reaction is more convenient and more efficient than previous methods which require prior conversion of carboxylic acids to acyl chlorides. The LC separation of amine derivatives has good reproducibility. The established method is also suitable for the determination of other amines in various biological fluids.
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