Carbapenemase Detection Among Carbapenem-Resistant Acinetobacter baumannii Clinical Isolates Using a Modified Blue-Carba Test

Abstract

Objective:Acinetobacter baumanniiis a Gram-negative opportunistic bacteria with carbapenemase-associated antibiotic resistance accounting for a majority of nosocomial infections globally. We sought to detectcarbapenemase producers among carbapenem-resistant A. baumannii(CRAB) clinical isolates using Blue-Carba Test(BCT).Methods: This study was an observational descriptive study. At first, A. baumanniiclinical isolates (142) were collected from January 2014 to September 2017and stored at the Microbiology Laboratoryof the Faculty of Medicine, Atma Jaya Hospital. Next step involved the identification of the carbapenem-resistant isolates using WHONET 5.3 software (https://whonet.org/software.html). Here, Imipenem and Meropenem served as markers. Finally, the identified carbapenem-resistant isolates were subjected to Blue-Carba Test. Results: Among 142 A. baumannii isolates, 51.4% (73/142) were identified as CRAB. Of these, 36 isolates were revived and all were found to be carbapenemase positive. Conclusion: Conclusively, a cent percent (36/36) prevalence of carbapenemase producers was observed in the CRAB group, which is an important clinical finding for antibiotic selection against A. baumannii infection.©2022iGlobal Research and PublishingFoundation. All rights reserved.