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Abstract
Carbachol produced an approximately 2-fold stimulation, with respect to basal, of [ 3H]inositol monophosphate ([ 3H]IP 1) accumulation in microdissected slices of rat substantia nigra pars reticulata (SNr), measured as a percentage of [ 3H]inositol incorporation. The response to carbachol was inhibited by 75 ± 7% by 1 μM pirenzepine, consistent with a muscarinic M 1 receptor-mediated response. Carbachol-induced [ 3H]IP 1 accumulation was not reduced by pretreatment of the SNr slices with NMDA, in contrast to the 85% inhibition observed in striatal slices. The results suggest that measurement of carbachol-induced phosphoinositide hydrolysis may be a feasible approach for studying muscarinic M 1 receptor function in slices of SNr.
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