Abstract
This study aimed to characterize rhizobia isolates of root and stem nodules of Discolobium spp., a native legume of Pantanal. Ten isolated rhizobia were randomly chosen from the culture collection of the Soil Microbiology Laboratory of Federal University of Mato Grosso. The parameters evaluated were: growth in YM medium with different salt concentrations (0%, 1.5% and 3%), different initial pH levels (6.8, 3.0 and 9.0), in two different temperatures (28 and 39 °C), and mucus production. The analyses showed that all isolates were tolerant to salinity and to temperature of 39 °C. The alkaline pH was more harmful than acidic pH to the growth of isolates, which may be related to adaptation to acid soils.
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