Abstract
Leaf anatomic characterization of populations of Bromus auleticus Trin. Ex Nees (Poaceae). This work was carried out to characterize the leaf anatomy of eight Bromus auleticus populations collected in Rio Grande do Sul, Brazil, and called A5, A6, A8, A17, A23, A26 and A27. Totally expanded leaves were collected from plants in the vegetative stage and were sectioned and stained with basic fuchsine and alcian blue. A single layer of epidermis with a smooth and fi ne cuticle on both surfaces, short tricomes, vascular bundles in three sizes, and the presence of cells with lignifi ed walls along the bundles and on the leaf edge were observed in all populations. The number of bulliform cells varied from three to fi ve, with the exception of population. A25, which ranged from fi ve to eight cells. Three populations presented long tricomes (A6, A23 and A27) and palisade chlorenchyma (A6, A7 and A26), fi ve had grooves on the leaf surface (A8, A17, A23, A26, A27) and homogeneous chlorenchyma (A5, A8, A17, A23, A27), two presented a large intercellular space in the mesofi le (A7 and A17), and two showed a more visible cuticle on the abaxial surface (A7 and A8). These variations could refl ect differences in relation to the nutritive value of the populations and be used in plant breeding programs for the genetic improvement of bromegrass.
Highlights
Could reflect differences in relation to the nutritive value of the populations and be used in plant breeding programs for the genetic improvement of bromegrass
As características anatômicas de folhas e caules podem ser utilizadas complementarmente como descritores de germoplasma de plantas forrageiras e no melhor entendimento da variabilidade temporal da qualidade nutricional das pastagens
Los recursos fitogeneticos del género Bromus en el Cono Sur. Procisur, Montevideo, Uruguay, p.7-18
Summary
O experimento foi conduzido na Universidade de Passo Fundo, região do Planalto Médio do Rio Grande do Sul, a 28°15’ de latitude sul e 52°24’ de longitude oeste, com altitude de aproximadamente 700m acima do nível do mar. As plantas foram estabelecidas, mediante mudas, em abril de 2002, em delineamento completamente casualizado, com dez repetições (plantas individuais) distantes 80cm entre si. Foram imediatamente fixadas em FAA 70 por aproximadamente 48h e conservadas em etanol 70oGL (Johansen, 1940). As secções transversais da lâmina foliar foram feitas à mão livre, coradas mediante a combinação de fucsina e azul de alcian (Luque et al, 1996) e montadas com glicerina 50%. Após a seleção das melhores lâminas foram feitas fotografias em fotomicroscópio Olympus BX50
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